
Prothrombin Time (PT) is one of the most frequently measured coagulation parameter. The PT Owren’s method is predominantly used in the Nordics. It is preferred over the internationally most used PT Quick method as it is
less sensitive to interferences.
For whole blood, plasma or capillary method, one- or two- components
For optical and mechanical detection systems
Distinct clot formation suitable for mechanical systems
Sensitive at remarkably low PT-activity (high INR)
General Description
MediRox Owren´s PT reagent GHI131-10 consist of lyophilised thromboplastin from rabbit brain powder and plasma fraction of bovine origin. The plasma fraction is to high degree deficient in FII, FVII and FX is the source of fibrinogen and FV. The reagent formula has been designed to create an extra strong clot, making it suitable for both mechanical and optical measuring systems.
Performance Characteristics
MRX PT Owren’s reagent is intended for the determination of Prothrombin Complex Activity and gives information about the activity of the vitamin K-dependent coagulation factors II, VII and X. A main reason for the preference for this method in Scandinavia is that the Owren’s method, in contrast to the more frequently used Quick method is less sensitive to interference by lupus antibodies, platelets or excess of citrate anticoagulant. The insensitivity to variations in Factor V and fibrinogen is also appreciated.
In addition, PT Owren’s can be measured in whole-blood or plasma, and since it employs a greater reagent-to-volume ratio, it is more suited for near-patient applications. Furthermore, the heparin insensitivity is better for the Owren’s method than the PT Quick method and to further increase the insensitivity to heparin, a polybrene buffer is available. Provided that results are expressed in INR, PT Quick and PT Owren’s give comparable results.
Sensitivity & Specificity
Details & typical data
Product form: | Lyophilised |
Available volumes: |
• 4 mL
• 10 mL • 20 mL |
Origin: | Rabbit brain thromboplastin and bovine plasma |
Additional products needed: |
• Diluent (GHI154) or CLSI CLRW type water or equivalent
• CaCl2 (GHI155) • PT Buffer (MRX150, MRX152) • Scandinavian Controls, L1/L2 (GHI163/GHI169, GHI162/GHI167B, GHI164/GHI170) • Eximius Control Basic or Plus (L1/L2/L3) (MRX170-MRX173, MRX180-MRX183) • Calibration according to local routines for PT Owren’s |
Range: | <10 INR |
Expected ISI value: | 0,9-1,2 |
Factor sensitivity: | FII, FVII and FX |
Stability & storage
Storage: | 2-8 °C |
Shelf-life: | 30 months at 2-8 °C |
Reconstituted solution: | 7 days at 2-25 °C |
Ordering information
Reference number | Product description | Pack size |
---|---|---|
GHI131-4 | MRX PT Owren's | 10x4 mL |
GHI131-10 | MRX PT Owren's | 10x10 mL |
GHI131-20 | MRX PT Owren's | 10x20 mL |
GHI155-2 | Calcium Chloride (CaCl2) | 10x2 mL |
GHI155-5 | Calcium Chloride (CaCl2) | 10x5 mL |
GHI155-10 | Calcium Chloride (CaCl2) | 10x10 mL |
GHI154-2 | Diluent (CLSI CLRW type water) | 10x2 mL |
GHI154-5 | Diluent (CLSI CLRW type water) | 10x5 mL |
GHI154-10 | Diluent (CLSI CLRW type water) | 10x10 mL |
MRX150 | PT Buffer | 10x10 mL |
MRX152 | PT Buffer (polybrene) | 10x10 mL |
GHI163/169 | Basic Normal/Abnormal Control | 10x1 mL pack per level |
GHI162/167B | Normal Abnormal Multi Control | 10x1 mL pack per level |
MRX170 | Eximius Control Basic, L1+L2+L3 | 4+4+2x1 mL |
MRX171/172/173 | Eximius Control Basic, L1/L2/L3 | 10x1 mL per pack |
MRX180 | Eximius Control Plus, L1+L2+L3 | 4+4+2x1 mL |
MRX181/182/183 | Eximius Control Plus, L1/L2/L3 | 10x1 mL per pack |
MRX PT Owren's product line

GHI131-10
MRX Owren's PT, 10mL10x10 mL

GHI131-4
MRX Owren's PT, 4 mL10x4 mL

GHI155-2
CaCl2, 2 mL10x2 mL

GHI155-5
CaCl2, 5 mL10x5 mL

GHI155
CaCl2, 100 mL6x100 mL
All Coagulation Reagents
MRX FPS
Latex-based liquid components for quantifying free Protein S in human citrated plasma using readers with wavelengths 600-800nm.
MRX Fib Clauss
Lyophilised reagents for quantifying fibrinogen in human citrated plasma, according to the Clauss method.
MRX AT
FXa-based chromogenic assay consisting of liquid components for quantifying functional Antithrombin activity in human citrated plasma.
MRX Thrombin Time
Two different lyophilised reagents for detection of fibrinogen abnormalities, and the presence of heparin and other thrombin inhibitors in human citrated plasma.
MRX APTT
Liquid compontent kits containing phospholipid extract and silica contact activator. Sensitive to heparin as well as factors and insensitive to lupus anticoagulant.
MRX D-Dimer
Latex-based liquid components for quantifying D-dimer fragments in human citrated plasma using readers with wavelengths 350-900nm.
MRX PT Quick
PT reagent from rabbit brain origin with an ISI ~1 for responsive determination of Prothrombin Time in citrated human plasma.
To order a desired product, send an email to order@medirox.se
or
If you have any further thoughts or questions, don’t hesitate to call us at +46 (0)155 45 44 10.