PT Quick is the internationally most common method to measure Prothrombin Time (PT), and is normally the assay of choice to monitor oral anticoagulat therapy. Moreover, PT Quick is used as a fundamental screening test for acquired or inherited bleeding disorders.
Low ISI ~1
Calibrated against WHO international reference preparation RBT/05
Applicable on optical and mechanical instruments
The PT is the method of choice for monitoring oral anticoagulant therapy and is a fundamental screening test for acquired or inherited bleeding disorders. During oral anti-coagulant therapy, the activity of vitamin K-dependant clotting factors (FII, FVII, FIX, FX, protein C and protein S) is reduced and PT time is increased thereby enable the quantitative determination of prothrombin activity.
MRX PT Quick is a highly sensitive diagnostic reagent intended for use in performing PT tests. MRX PT Quick reagent contains a source of rabbit tissue thromboplastin and calcium that specifically activate FVII in the extrinsic coagulation pathway. MRX PT Quick reagent is easy to reconstitute, calcium is already added to the reagent before lyophilisation. Calibration was performed against the most recent WHO thromboplastin standard, RBT/05, which is traceable to the first reference preparation of thromboplastins. The calibration assures low lot-to-lot variation and consequently results in reliable PT determination.
PT Quick results are expressed in either seconds, percent activity or international normalised ratio, INR. The international recommendations of reporting the PT time is INR and refers to the international sensitivity index (ISI) of thromboplastins. INR is calculated from the quotient between the coagulation time for sample and the coagulation time for a normal plasma (MNPT) raised to the method’s ISI-value (International Sensitivity Index).
INR = (PT/MNPT)ISI
Sensitivity & specificity
Details & typical data
• 2 mL
• 4 mL
• 5 mL
• 10 mL
|Origin:||Rabbit brain thromboplastin|
|Additional products needed:||
• Diluent (GHI154) or CLSI CLRW type water or equivalent
• Eximius Control Basic or Plus (L1/L2/L3) (MRX170-173, MRX180-183)
• Multi Calibrator II (MRX1203) or PT 6-Level Calibrator (MRX1205)
|Expected ISI value:||0,9 - 1,2|
|Factor sensitivity:||FI, FII, FV, FVII and FX|
|No interferences with:||Heparin UFH <2 U/mL, Triglycerides <5 g/L, Bilirubin <500 mg/L, Hemoglobin <5 g/L|
Stability & storage
|Shelf-life:||24 months at 2-8 °C|
|Reconstituted solution:||5 days at 2-25 °C|
|Reference number||Product description||Pack size|
|MRX943-2||MRX PT Quick||10x2 mL|
|MRX943-4||MRX PT Quick||10x4 mL|
|MRX943-5||MRX PT Quick||10x5 mL|
|MRX943-10||MRX PT Quick||10x10 mL|
|MRX170||Eximius Control Basic L1+L2+L3||4+4+2x1 mL|
|MRX171/172/173||Eximius Control Basic L1/L2/L3||10x1 mL pack per level|
|MRX180||Eximius Control Plus L1+L2+L3||4+4+2x1 mL|
|MRX181/182/183||Eximius Control Plus L1/L2/L3||10x1 mL pack per level|
|MRX1203||Multi Calibrator II (AT, fibrinogen, PT Quick)||1x1 mL|
|MRX1203-10||Multi Calibrator II (AT, fibrinogen, PT Quick)||10x1 mL|
|MRX1205||PT 6-Level Calibrator (INR 1-6)||6x1 mL|
|GHI154-2||Diluent (CLSI CLRW type water)||10x2 mL|
|GHI154-4||Diluent (CLSI CLRW type water)||10x4 mL|
|GHI154-5||Diluent (CLSI CLRW type water)||10x5 mL|
|GHI154-10||Diluent (CLSI CLRW type water)||10x10 mL|
MRX PT Quick product line
MRX943-2PT Quick, 2 mL
MRX943-5PT Quick, 5 mL
MRX943-10PT Quick, 10 mL
MRX943-4PT Quick, 4 mL
MRX171Eximius Control Basic L1
MRX172Eximius Control Basic L2
MRX173Eximius Control Basic L3
MRX181Eximius Control PLUS L1
MRX182Eximius Control PLUS L2
MRX183Eximius Control PLUS L3
All Coagulation Reagents
Latex-based liquid components for quantifying free Protein S in human citrated plasma using readers with wavelengths 600-800nm.
MRX Fib Clauss
Lyophilised reagents for quantifying fibrinogen in human citrated plasma, according to the Clauss method.
FXa-based chromogenic assay consisting of liquid components for quantifying functional Antithrombin activity in human citrated plasma.
MRX Thrombin Time
Two different lyophilised reagents for detection of fibrinogen abnormalities, and the presence of heparin and other thrombin inhibitors in human citrated plasma.
Liquid compontent kits containing phospholipid extract and silica contact activator. Sensitive to heparin as well as factors and insensitive to lupus anticoagulant.
Latex-based liquid components for quantifying D-dimer fragments in human citrated plasma using readers with wavelengths 350-900nm.
MRX PT Owren’s
The predominantly used PT method in the Nordics as it is less sensitive to interferences. The assay can be used with both whole blood and plasma.