MRX FPS consists of sub-micron sized polystyrene particles coupled to two different monoclonal antibodies specific for protein S. This system is not sensitive to protein S bound to C4b-binding protein, but only reacts with the free active form of protein S. When the reagent is exposed to a plasma sample containing free Protein S, the particles will agglutinate, giving rise to increased light-scattering. MRX FPS is suitable for automatic coagulation and clinical chemistry instruments using turbidimetric detection in the 600 – 800 nm wavelength range.
Latex based assay
Specific for free protein S
Good correlation to existing Free Protein S assays
Multiplatform with applications for most coagulation instruments
Inherited and acquired protein S deficiency is an important risk factor for venous thrombosis. Measurements of free protein S can better discriminate between the different subtypes of Protein S deficiency than what measurement of only total protein S concentrations can and has therefore become an important tool in clinical thrombosis investigations.
MRX FPS is used for quantitative determination of FPS in human plasma. Quantitative determination of FPS in human plasma is used in clinical investigations of different diseases, e.g acquired Protein S deficiency which can be caused by several disorders and treatments. Protein S is a vitamin K dependent glycoprotein with anticoagulant properties and in the presence of calcium, Protein S
acts as a cofactor to and forms a complex with activated protein C (APC). The complex adheres to negatively charged phospholipid membranes which increases the anticoagulant activity of APC. The normal total concentration of Protein S in human plasma is in the range 20 – 25 mg/L; approximately two thirds of this is bound to C4b-binding protein (C4BP), whereas the remaining non-bound fraction is FPS.
Sensitivity & specificity
Details & typical data
|Product form:||Liquid components|
• MRX153: 2.5 mL latex + 4 mL reaction buffer
• Other filling volumes upon request
|Additional products needed:||
• Eximius Control Plus (L1/L2/L3) (MRX180-MRX183)
• Scandinavian Controls Multi Plus (GHI164/GHI170)
• FPS Calibrator (MRX1206)
• Sample Diluent (0,9 % NaCl) for calibrator dilutions (MRX184)
• Diluent (GHI154)
|Linear range:||20-150 (FPS %)|
Intra-device (CV %):
• L1: 4.01
• L2: 2.83
• L3: 3.01
|Interfering substances:||No interferences with UF and LMW Heparin <100 U/mL, Bilirubin < 0,8 g/L, Triglycerides <30 g/L, Hemoglobin <10 g/L|
Stability & storage
|Shelf-life:||18 months at 2-8 °C|
|Open vial stability:||4 weeks at 2-8 °C / 4 weeks at 2-25 °C|
|Reference number||Product description||Pack size|
|MRX153||MRX FPS (latex + reaction buffer)||3x2.5 mL + 3x4 mL|
|MRX180||Eximius Control Plus, L1+L2+L3||4+4+2x1 mL|
|MRX181/182/183||Eximius Control Plus L1/L2/L3,||10x1 mL pack per level|
|GHI164/170||Scandinavian Control Multi Plus, L1/L2||10x1 mL pack per level|
|MRX1206||FPS Calibrator||1x1 mL|
|MRX184||Sample Diluent (0,9 % NaCl)||10x8 mL|
MRX FPS product line
MRX153600-800 nm FPS Kit
3x2,5 mL Latex
3x4 mL Reaction Buffer
Sample Diluent (0,9% NaCl) for purpose of dilution of test plasmas and calibrator plasmas for the Fib Clauss, Antithrombin and D-Dimer assays.
Diluent, for purpose of reconstitution of lyophilised coagulation reagents and controls, where water of a high purity grade is required and the addition of 4 mM NaN3 is not disturbing.
10x2 mL (GHI154-2)
10x4 mL (GHI154-4)
10x5 mL (GHI154-5)
10x10 mL (GHI154-10)
MRX180Eximius Control PLUS Triple Set
4x1 mL L1
4x1 mL L2
2x1 mL L3
MRX181Eximius Control PLUS L1
MRX182Eximius Control PLUS L2
MRX183Eximius Control PLUS L3
GHI170Scandinavian Multi Control PLUS, abnormal
GHI164Scandinavian Multi Control PLUS, normal
All Coagulation Reagents
Liquid compontent kits containing phospholipid extract and silica contact activator. Sensitive to heparin as well as factors and insensitive to lupus anticoagulant.
Latex-based liquid components for quantifying D-dimer fragments in human citrated plasma using readers with wavelengths 350-900nm.
MRX PT Owren’s
The predominantly used PT method in the Nordics as it is less sensitive to interferences. The assay can be used with both whole blood and plasma.
MRX Thrombin Time
Two different lyophilised reagents for detection of fibrinogen abnormalities, and the presence of heparin and other thrombin inhibitors in human citrated plasma.
MRX Fib Clauss
Lyophilised reagents for quantifying fibrinogen in human citrated plasma, according to the Clauss method.
FXa-based chromogenic assay consisting of liquid components for quantifying functional Antithrombin activity in human citrated plasma.
MRX PT Quick
PT reagent from rabbit brain origin with an ISI ~1 for responsive determination of Prothrombin Time in citrated human plasma.