Fibrinogen Clauss (Fib Clauss) is the internationally most established method to determine fibrinogen in human plasma, which is of clinical importance when unexplained bleeding occurs. To fullfill the market requirements and needs MediRox has developed two different designs of Fib Clauss reagents, one having 100 UNIH/mL thrombin being highly resistent to dabigatran and one having 29 UNIH/mL thrombin suitable for the more price sensitive markets where negigible amount dabigatran is prescribed.
Two Fib Clauss products (100 UNIH/mL and 29 UNIH/mL bovine thrombin)
Linearity up to 8 g/L
MRX Fib Clauss is a functional assay used for the quantitative measurement of fibrinogen in human citrated plasma according to the Clauss technique, where a high concentration of thrombin is added to diluted plasma and the clotting time is measured. The result is compared with a calibration curve using a reference plasma with known fibrinogen concentrations, and a result in g/L or mg/dL is obtained.
Sensitivity & Specificity
Details & typical data
|Fib Clauss products:||
• MRX942 (29 UNIH/mL thrombin)
• MRX942B (100 UNIH/mL thrombin)
• 2 mL for both MRX942 and MRX942B
• 5 mL for both MRX942 and MRX942B
|Additional products needed:||
• Diluent (GHI154) or CLSI CLRW type water or equivalent
• Sample Diluent (0,9% NaCl) for sample and calibrator dilutions (MRX184)
• Eximius Control Basic or Plus (L1/L2/L3) (MRX170-MRX173, MRX180-MRX183)
• Scandinavian Controls Basic, Multi and Multi Plus (L1/L2) (GHI163/GHI169; GHI164/GHI170; GHI164 GHI170)
• Fib Clauss Calibrator (MRX1204), Multi Calibrator I (MRX1202) or Multi Calibrator II (MRX1203)
• Imidazol Buffer (MRX185-15) required for some instruments
|Linearty:||Linear calibration from 0,6-6,0 g/L||Linear calibration from 0,6-8,0 g/L|
|Expected values:||1,59-3,51 g/L||1,59-3,51 g/L|
|Interfering substances:||No interferences with Bilirubin <50 mg/L, Heparin <1,0 IU/mL, Triglycerides <3,75 g/L, Hemoglobin <0,375 g/L, Dabigatran <100 μg/L||No interferences with Bilirubin <50 mg/L, Heparin <4,0 IU/mL, Triglycerides <3,75 g/L, Dabigatran <475 μg/L, Haemolyzed plasma should not be assayed|
Stability & storage
|Shelf-life:||24 months at 2-8 °C|
|Reconstituted solution:||7 days at 2-25 °C|
|Reference number||Product description||Size|
|MRX942B-2||MRX Fib Clauss, 100 UNIH/mL||10x2 mL|
|MRX942B||MRX Fib Clauss, 100 UNIH/mL||10x5 mL|
|MRX942-2||MRX Fib Clauss, 29 UNIH/mL||10x2 mL|
|MRX942||MRX Fib Clauss, 29 UNIH/mL||10x5 mL|
|MRX170||Eximius Control Basic, L1+L2+L3||4+4+2x1 mL|
|MRX171/172/173||Eximius Control Basic, L1/L2/L3||10x1 mL pack per level|
|MRX180||Eximius Control Plus, L1+L2+L3||4+4+2x1 mL|
|MRX181/182/183||Eximius Control Plus, L1/L2/L3||10x1 mL pack per level|
|GHI163/169||Scandinavian Control Basic, L1/L2||10x1 mL pack per level|
|GHI162/167B||Scandinavian Control Multi, L1/L2||10x1 mL pack per level|
|GHI164/170||Scandinavian Control Multi Plus, L1/L2||10x1 mL pack per level|
|MRX1204||Fib Clauss Calibrator||1x1 mL|
|MRX1202||Multi Calibrator I (AT, fibrinogen, D-dimer)||1x1 mL|
|MRX1203||Multi Calibrator II (PT Quick, AT, fibrinogen)||1x1 mL|
|GHI154||Diluent (CLSI CLRW type water)||10x5 mL|
|MRX184||Sample Diluent (0,9 % NaCl)||10x8 mL|
|MRX185-15||Imidazol Buffer||10x15 mL|
MRX Fib Clauss product line
MRX942-2MRX Fib Clauss (29), 2 mL
MRX942-5MRX Fib Clauss (29), 5 mL
MRX942B-5MRX Fib Clauss (100), 5 mL
MRX171Eximius Control Basic L1
MRX172Eximius Control Basic L2
MRX173Eximius Control Basic L3
MRX181Eximius Control Plus L1
MRX182Eximius Control Plus L2
MRX183Eximius Control Plus L3
Multi Calibrator I (AT, Fib, DD) intended for calibration of AT, Fib Clauss and D-Dimer assays.
1x1 mL (MRX1202)
10x1 mL (MRX1202-10)
Multi Calibrator II (AT, Fib, PT) intended for calibration of AT, Fib Clauss and PT assays. PT is obtained as PT %.
1x1 mL (MRX1203)
10x1 mL (MRX1203-10)
All Coagulation Reagents
MRX FDP is a latex immunoassay for quantifying fibrin and fibrinogen degradation products in human plasma using readers with wavelengths 500-650nm.
Liquid compontent kits containing phospholipid extract and silica contact activator. Sensitive to heparin as well as factors and insensitive to lupus anticoagulant.
FXa-based chromogenic assay consisting of liquid components for quantifying functional Antithrombin activity in human citrated plasma.
MRX PT Quick
PT reagent from rabbit brain origin with an ISI ~1 for responsive determination of Prothrombin Time in citrated human plasma.
Latex-based liquid components for quantifying free Protein S in human citrated plasma using readers with wavelengths 600-800nm.
Latex-based liquid components for quantifying D-dimer fragments in human citrated plasma using readers with wavelengths 350-900nm.
MRX PT Owren’s
The predominantly used PT method in the Nordics as it is less sensitive to interferences. The assay can be used with both whole blood and plasma.
MRX Thrombin Time
Two different lyophilised reagents for detection of fibrinogen abnormalities, and the presence of heparin and other thrombin inhibitors in human citrated plasma.