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FXa-based chromogenic assay consisting of liquid components for quantifying functional Antithrombin activity in human citrated plasma.

Antithrombin (AT) activity is measured in citrated plasma for diagnosis of AT deficiency. MediRox AT liquid reagent is a ready-to-use chromogenic kit based on bovine FXa and chromogenic FXa substrate.

Liquid components, ready to use

FXa-based AT reagents

Insensitiv to Heparin Cofactor II (HCII)

Detects both type I and type II AT deficiencies


General Description

AT is the primary physiological inhibitor of thrombin and FXa in plasma, and thereby effectively regulates blood coagulation. An inherited AT deficiency is often associated with thromboembolic disease, and acquired deficiencies may occur in liver disease and disseminated intravascular coagulation.

Performance Characteristics

AT activity is determined in a two-stage chromogenic assay as follows:

An excess of FXa is added to citrated plasma in the presence of heparin. The residual FXa activity is determined from the hydrolysis of a chromogenic FXa Substrate, which results in the release of free p-nitroaniline (pNA). The amount of pNA is measured by absorbance at 405 nm and the result is inversely proportional to the amount of AT present in citrated plasma, and expressed as % AT activity.

Hep + citrated plasma (AT) + FXa (excess) —–> Hep-AT-FXa + FXa residual

FXa residual + Chromogenic Substrate      —–> pNA + peptide

Sensitivity & specificity

Details & typical data

Product form: Liquid components
Available volumes: • FXa reagent: 6 mL
• Chromogenic substrate: 3 mL
• Other filling volumes available upon request
Origin: Bovine FXa
Additional products needed: • Sample Diluent, 0,9 % NaCl (MRX184)
• Eximius Control Basic or Plus (L1/L2/L3) (MRX170-MRX173, MRX180-MRX183)
• Scandinavian Controls Basic, Multi and Multi Plus (L1/L2) (GHI163/GHI169; GHI164/GHI170; GHI164 GHI170)
• AT Calibrator (MRX1201), Multi Calibrator I (MRX1202) or Multi Calibrator II (MRX1203)
Linearity: 0,8-1,20 IU/mL
Interfering substances: Not affected by the possible interferents:
UFH and LMW heparin <4,0 IU/mL; Bilirubin <0,4 g/L; triglycerides <5 g/L; hemoglobin <1,5 g/L

Stability & storage

Storage: 2-8 °C
Shelf-life: 24 months at 2-8 °C
Open vial stability: 30 days at 2-25 °C

Ordering information

Reference number Product description Pack size
MRX1200 Antithrombin FXa (reagent + substrate) 6x6 mL FXa + 3x3 mL substrate
MRX170 Eximius Control Basic, L1+L2+L3 4+4+2x1 mL
MRX171/172/173 Eximius Control Basic, L1/L2/L3 10x1 mL pack per level
MRX180 Eximius Control Plus, L1+L2+L3 4+4+2x1 mL
MRX181/182/183 Eximius Control Plus, L1/L2/L3 10x1 mL pack per level
GHI163/169 Scandinavian Control Basic, L1/L2 10x1 mL pack per level
GHI162/167B Scandinavian Control Multi, L1/L2 10x1 mL pack per level
GHI164/170 Scandinavian Control Multi Plus, L1/L2 10x1 mL pack per level
MRX1201 AT Calibrator 1x1 mL
MRX1202 Multi Calibrator I (AT, fibrinogen, D-dimer) 1x1 mL
MRX1203 Multi Calibrator II (PT Quick, AT, fibrinogen) 1x1 mL
MRX184 Sample Diluent (0,9% NaCl) 1x8 mL
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