MRX PT Owren's

The predominantly used PT method in Scandinavia due to the high sensitivity to interferences. The assay can be used with both whole blood and plasma.

Prothrombin Time (PT) is one of the most frequently measured/analysed coagulation parameter. The Owren’s PT method is predominantly used in Scandinavia, Japan and parts of Benelux and Austria, where it is preferred over PT Quick method since it is less sensitive to interferences, and can be used with both whole blood and plasma.

For whole blood, plasma or capillary method, one- or two- components

For optical and mechanical detection systems

Distinct clot formation suitable for mechanical systems

Sensitive at remarkably low PT-activity (high INR)

 

General Description

MediRox Owren´s PT reagent GHI131-10 consist of lyophilised thromboplastin from rabbit brain powder and plasma fraction of bovine origin. The plasma fraction is to high degree deficient in FII, FVII and FX is the source of fibrinogen and FV. The reagent formula has been designed to create an extra strong clot, making it suitable for both mechanical and optical measuring systems.

PT Owren´s vs PT Quick pdf

Performance Characteristics

MRX PT Owren’s reagent is intended for the determination of Prothrombin Complex Activity and gives information about the activity of the vitamin K-dependent coagulation factors II, VII and X. A main reason for the preference for this method in Scandinavia is that the Owren’s method, in contrast to the more frequently used Quick method is less sensitive to interference by lupus antibodies, platelets or excess of citrate anticoagulant. The insensitivity to variations in Factor V and fibrinogen is also appreciated.

 

In addition, PT Owren’s can be measured in whole-blood or plasma, and since it employs a greater reagent-to-volume ratio, it is more suited for near-patient applications. Furthermore, the heparin insensitivity is better for the Owren’s method than the PT Quick method and to further increase the insensitivity to heparin, a polybrene buffer is available. Provided that results are expressed in INR, PT Quick and PT Owren’s give comparable results.

Sensitivity & Specificity

Details & typical data

Product form: Lyophilised
Available volumes: • 4 mL
• 10 mL
• 20 mL
Origin: Rabbit brain thromboplastin and bovine plasma
Additional product requirements: • Diluent (GHI154) or CLSI CLRW type water or equivalent
• CaCl2 (GHI155)
• PT Buffer (MRX150, MRX152)
• Scandinavian Controls, L1/L2 (GHI163/GHI169, GHI162/GHI167B, GHI164/GHI170)
• Eximius Control Basic or Plus (L1/L2/L3) (MRX170-MRX173, MRX180-MRX183)
• Calibration according to local routines for PT Owren’s
Range: <10 INR
Expected ISI value: 0,9-1,2
Factor sensitivity: FII, FVII and FX

Stability & storage

Storage: 2-8 °C
Shelf-life: 24 months at 2-8 °C
Reconstituted solution: 7 days at 2-25 °C

Ordering information

Reference number Product description Pack size
GHI131-4 MRX PT Owren's 10x4 mL
GHI131-10 MRX PT Owren's 10x10 mL
GHI131-20 MRX PT Owren's 10x20 mL
GHI155-2 Calcium Chloride (CaCl2) 10x2 mL
GHI155-5 Calcium Chloride (CaCl2) 10x5 mL
GHI155-10 Calcium Chloride (CaCl2) 10x10 mL
GHI154-2 Diluent (CLSI CLRW type water) 10x2 mL
GHI154-5 Diluent (CLSI CLRW type water) 10x5 mL
GHI154-10 Diluent (CLSI CLRW type water) 10x10 mL
MRX150 PT Buffer 10x10 mL
MRX152 PT Buffer (polybrene) 10x10 mL
GHI163/169 Basic Normal/Abnormal Control 10x1 mL pack per level
GHI162/167B Normal Abnormal Multi Control 10x1 mL pack per level
MRX170 Eximius Control Basic, L1+L2+L3 4+4+2x1 mL
MRX171/172/173 Eximius Control Basic, L1/L2/L3 10x1 mL per pack
MRX180 Eximius Control Plus, L1+L2+L3 4+4+2x1 mL
MRX181/182/183 Eximius Control Plus, L1/L2/L3 10x1 mL per pack
 
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