Thrombin Time (TT) is a common clotting test used to detect fibrinogen abnormalities, and the presence of heparin and other thrombin inhibitors in human citrated plasma. In order to fulfil these features, MRX TT reagent comes with two reconstitution volumes, 2 mL for heparin detection and 5 mL for the detection of fibrinogen malfunction.
High precision, CV ≤2,1 % within and between runs
One vial reagent with multiple reconstitution volumes (2 or 5 mL of distilled water)
Suitable for optical and mechanical instruments
The TT assay is performed to study a patient’s fibrin polymerisation, for the evaluation of disseminated intravascular coagulation, and for hereditary fibrinogen abnormalities. A prolonged thrombin time is usually seen in patient plasma with low fibrinogen concentration, and in patient plasma with defect in fibrinogen cleavage. TT might be used for the evaluation of plasmas with prolonged APTT. MRX TT is affected by fibrinogen inhibitors such as heparin, hirudin etc.
Plasma fibrinogen is cleaved by thrombin to form fibrin, which polymerises to form a fibrin clot. Fibrinogen present in the sample is converted to fibrin by the addition of purified bovine thrombin and the time (measured in seconds) to form a clot is reported as the thrombin time.
MRX TT utilises equal parts of reagent and sample when screening for the presence of heparin as well as fibrinogen malfunction. An abnormal clotting time interval could indicate fibrinogen deficiency, hypofibrinogenemia, heparin therapy or dabigatran therapy.
Sensitivity & Specificity
Details & Typical Data
|Sensitivity:||MRX941 (2 mL) is sensitive for heparin in the range 0,03‑0,25 IU/mL|
|Normal Range:||MRX941 (2 mL) 7,8 sec; MRX941 (5 mL) 17,1 sec|
|Interfering substances:||Thrombin time results may be affected (results in prolonged clotting time) by many commonly administered drugs. For example heparin, and direct thrombin inhibitors (DTI) such as hirudin and dabigatran, and other factor II inhibitor drugs. Thrombin time is also affected by haemolysis and high bilirubin concentration.|
Stability & Storage
|Shelf life:||24 months at 2-8 ºC|
|Reconstituted solution:||8 days at 2-8 °C 3 days at 15-25 °C|
|Reference Number||Product Description||Pack Size|
|MRX941||MRX Thrombin Time||10x5mL|
|MRX171||Eximius Control Basic L1||10x1 mL|
|MRX181||Eximius Control PLUS L1||10x1 mL|
|GHI154||Diluent (pur. water, reconst.)||10x8 mL|
MRX Thrombin Time product line
MRX941MRX Thrombin Time
MRX171Eximius Control Basic L1
MRX181Eximius Control PLUS L1
All Coagulation Reagents
Liquid compontent kits containing phospholipid extract and silica contact activator. Sensitive to heparin as well as factors and insensitive to lupus anticoagulant.
Latex-based liquid component kits for quantifying D-dimer fragments in human citrated plasma using readers with wavelengths 350-900nm.
MRX Owren’s PT
The predominantly used PT method in Scandinavia due to the high sensitivity to interferences. The assay can be used with both whole blood and plasma.
MRX Fib Clauss
Lyophilised reagents for quantifying fibrinogen in human citrated plasma, according to the Clauss method.
FXa-based chromogenic assay consisting of liquid components for quantifying functional Antithrombin activity in human citrated plasma.
MRX PT Quick
PT reagent from rabbit brain origin with an ISI ~1 for responsive determination of Prothrombin Time in citrated human plasma.